Studies on Riboflavin Deficiency in Tetrahymena pyriformis
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چکیده
منابع مشابه
Studies of Membrane Formation in Tetrahymena Pyriformis
A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...
متن کاملRespiratory Enzyme Studies in Tetrahymena pyriformis
Meyerhof, 0. (1945). J. biol. Chem. 157, 105. Meyerhof, 0. & Kaplan, A. (1951). Arch. Biochem. Biophy8. 38, 282. Meyerhof, 0. & Oesper, P. (1947). J. biol. Chem. 170, 1. Meyerhof, 0. & Wilson, J. R. (1949). J. biol. Chem. 180, 575. Nelson, N. (1944). J. biol. Chem. 153, 373. Nilsson, R. & Alm, F. (1941). Biochem. Z. 308, 262. Prankerd, T. A. J. & Altman, K. I. (1954). Biochem. J. 58, 622. Roe, ...
متن کاملStudies on Nuclear Structure and Function in Tetrahymena Pyriformis
This report describes a rapid, efficient method for isolating macronuclei from Tetrahymena. The macronuclear fraction contains only small amounts of micronuclear material and little detectable whole cell or cytoplasmic contamination. A method is also described for preparing a "micronuclear fraction" which contains 20-40 micronuclei for every macronucleus present. Electron microscope observation...
متن کاملRibosome Synthesis in Tetrahymena Pyriformis
The cellular site of synthesis of ribosomal RNA in Tetrahymena pyriformis was studied by analyzing the purified nuclear and cytoplasmic RNA from cells pulse labeled with uridine-(3)H. The results of studies using zonal centrifugation in sucrose density gradients show that the ribosomal RNA is synthesized in the nucleus as a large precursor molecule sedimenting at 35S. The 35S molecule undergoes...
متن کاملStudies of Native Glycogen Isolated from Synchronized Tetrahymena Pyriformis (hsm)
Native glycogen was isolated from Tetrahymena pyriformis (HSM) by isopycnic centrifugation in cesium chloride density gradients. A density of 1.62 to 1.65 was isopycnic for glycogen. Most of the banded glycogen existed as 35 to 40 mmicro particles which had a sedimentation coefficient of 214. These particles were composed of aggregates of 2 to 3 mmicro spherical particles. Extraction of glycoge...
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ژورنال
عنوان ژورنال: The Journal of Nutrition
سال: 1972
ISSN: 0022-3166,1541-6100
DOI: 10.1093/jn/102.9.1209